| Hugh Jackman - Logan / Wolverine |
| Halle Berry - Ororo Munroe / Storm |
| Ian McKellen - Eric Lehnsherr / Magneto |
| Patrick Stewart - Charles Xavier / Professor X |
| Famke Janssen - Jean Grey / Phoenix |
| Anna Paquin - Marie / Rogue |
| Kelsey Grammer - Dr. Henry 'Hank' McCoy / Beast |
| James Marsden - Scott Summers / Cyclops |
| Rebecca Romijn - Raven Darkholme / Mystique |
| Shawn Ashmore - Bobby Drake / Iceman |
| Aaron Stanford - John Allerdyce / Pyro |
| Vinnie Jones - Cain Marko / Juggernaut |
| Ellen Page - Kitty Pryde |
| Daniel Cudmore - Peter Rasputin / Colossus |
| Ben Foster - Warren Worthington III / Angel |
| Michael Murphy - Warren Worthington II |
| Dania Ramirez - Callisto |
| Shohreh Aghdashloo - Dr. Kavita Rao |
| Josef Sommer - The President |
| Bill Duke - Trask |
| Eric William Dane - Multiple Man |
| Desiree Zurowski - Mrs. Grey |
| Adrian Hough - Mr. Grey |
| Haley Ramm - Young Jean Grey |
| Chris Claremont - Lawnmower Man |
| Stan Lee - Waterhose Man |
| Cayden Boyd - Young Angel |
| Tanya Newbould - Dr. McCoy's Assistant |
| Anthony Heald - FBI Mystique Interrogator |
| Cameron Bright - Jimmy / Leech |
| Connor Widdows - Jones |
| Kea Wong - Jubilation Lee / Jubilee |
| Shauna Kain - Theresa Rourke Cassidy / Siryn |
| Luke Pohl - Flea |
| Julian Richings - Mutant Theatre Organizer |
| Lloyd Adams - Lizard Man |
| Richard Yee - Little Phat |
| Via Saleaumua - Phat |
| Meiling Melançon - Psylocke |
| Omahyra Mota - Arclight |
| Clayton Watmough - Glob Herman |
| Ken Leung - Kid Omega |
| Julian Christopher - Prison Truck Guard #1 |
| Brad Kelly - Prison Truck Guard #2 |
| Makenzie Vega Norfolk - Prison Truck Little Girl |
| Mi-Jung Lee - Newscaster |
| Benita Ha - Worthington Technician |
| Ron James - Prison Truck Driver |
| R. Lee Ermey - Sergeant (voice) |
| Lance Gibson - Spike |
| Aaron Pearl - Team Leader |
| Chelah Horsdal - Minivan Mother |
| John Pyper-Ferguson - Minivan Father |
| Brenna O'Brien - Minivan Daughter |
| Justin Callan - Minivan Son #1 |
| Alex Ferris - Minivan Son #2 |
| Peter Kawasaki - Photographer |
| Ron Blecker - Commander |
| Emy Aneke - Alcatraz Lieutenant |
| David Smith - Omega Mutie |
| Olivia Williams - Dr. Moira MacTaggert |
